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Chymase is an enzyme that generates angiotensin II, which is a potent vasoconstrictor. It has been suggested that the level of chymase activity may be important in the regulation of fluid balance in the submandibular gland. In order to determine the possible role of chymase in fluid regulation, we investigated the effect of human recombinant growth hormone (rhGH) on the number of chymase-containing cells in dispersed submandibular gland acinar cell cultures. Fifteen young adult male rats were divided into three groups: the vehicle group, the rhGH group, and the positive control group. Rats in the rhGH and positive control groups were injected with 0.1 mg/kg and 2.0 microg/kg of rhGH, respectively, for 4 days. Rats in the vehicle group were injected with saline. At the end of the treatment period, each animal was killed, and its submandibular gland was immediately dissected. After isolation, the cells were dispersed and then plated in a defined serum-free medium. Chymase-containing cell number was evaluated using immunocytochemistry with an anti-chymase antibody. In addition to the positive controls, there was an increase in the number of chymase-containing cells in the rhGH group. When the saline group and the rhGH group were compared, chymase-containing cell number was significantly increased in the rhGH group (P Q:
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