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Qaidi (1984) Sajna - Music Video. Qaidi (1984) with song "Jab Pyar Ho Tha Aa Gaye" Mp3.Enhanced proteolytic degradation of N-myc downstream-regulated gene product by c-Jun N-terminal kinase 2 contributes to the downregulation of N-myc downstream-regulated gene product by protein phosphatase 2A in neuroblastoma cells.
We previously showed that protein phosphatase 2A (PP2A) dephosphorylates and downregulates N-myc downstream-regulated gene product (NDRG1) by enhancing its proteolytic degradation in neuroblastoma cells. In this study, we found that protein phosphatase 2A (PP2A) enhanced the expression of NDRG1 through proteolytic degradation. Treatment of neuroblastoma cells with cycloheximide enhanced NDRG1 expression, whereas treatment with calyculin A or okadaic acid suppressed NDRG1 expression. We found that knockdown of NDRG1 by RNA interference enhanced cycloheximide-induced c-Jun N-terminal kinase 2 (JNK2) expression, but not extracellular signal-regulated kinase 1 or 2 expression. Treatment with calyculin A suppressed NDRG1 expression by enhancing JNK2 expression, and conversely, treatment with okadaic acid suppressed JNK2 expression and enhanced NDRG1 expression. These findings strongly suggest that the enhancement of NDRG1 expression by PP2A through proteolytic degradation is related to the suppression of JNK2 by PP2A. Treatment with JNK inhibitors SP600125 or SP600125 + SB203580 suppressed cycloheximide-induced JNK2 expression and enhanced NDRG1 expression. NDRG1 was predominantly localized in the nucleus, and treatment with cycloheximide enhanced the nuclear translocation of NDRG1. These results indicate that NDRG1 is mainly localized in the nucleus, and treatment with cycloheximide enhances its nuclear translocation and the downregulation of NDRG1 by PP2A through proteolytic degradation.# -*- coding: utf-8 -* 0b46394aab
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